Windows 10 Superlite Install: Mpb Blastx

Use Rufus (free, reliable).

Because Superlite is stripped-down, follow these preparatory steps:

If you cannot get BLAST+ to run on your specific Superlite build (rare but possible), consider:


To run blastx from anywhere, add BLAST to your PATH: mpb blastx windows 10 superlite install

Test in a new Command Prompt:

blastx -version

If you see version info, success. If not, Superlite may be missing vcruntime140.dll – reinstall VC++ Redist.


Upon reboot, remove the USB drive. You will notice: Use Rufus (free, reliable)

Create a batch script run_blastx.bat:

@echo off
set BLASTDB=C:\Bioinformatics\db
set THREADS=2
set QUERY=%1
set OUTPUT=%2

if "%QUERY%"=="" ( echo Usage: run_blastx.bat query.fasta output.txt exit /b )

C:\Bioinformatics\blast\blastx.exe -query %QUERY% -db swissprot -out %OUTPUT% -outfmt 6 -num_threads %THREADS% echo BLASTX complete on Superlite. To run blastx from anywhere, add BLAST to your PATH:

Run with:

run_blastx.bat my_genes.fasta my_results.txt