Narishige Pc10 Manual New Direct
Narishige International (Narishige Group) still supports legacy products. While the PC-10 is discontinued, they often retain PDF archives.
The Narishige PC-10 is a classic workhorse, not a modern marvel. The new manual is clear, safety-conscious, and accurate. For the price, nothing else matches its mechanical simplicity. However, in 2026, most electrophysiology labs prefer a used Sutter P-97 over a new PC-10. But if you value repairability, no firmware, and immediate usability, the PC-10 remains a solid choice.
Rating:
Overall: 7.4/10 – Recommended for teaching & basic patch-clamp; not for advanced research.
Narishige PC-10 Go to product viewer dialog for this item. is a dual-stage, vertical glass micropipette puller designed for high-precision laboratory applications such as patch clamping and microinjection. While it has been largely superseded by the digital
, the PC-10 remains a robust tool for creating tips ranging from sub-micron sizes to tens of microns. Core Functionality and Modes
The PC-10 utilizes gravity-fed vertical pulling to ensure uniformity. It features a mode-selector knob that allows users to toggle between four primary operations: STEP 1 (Single-Stage):
Pulls the glass capillary in one continuous stretch. This mode is typically used to create longer, more supple pipettes with larger tip diameters. STEP 2 (Two-Stage):
Automates a mid-process change in settings. The first pull thins the glass, and the second pull finishes the tip, resulting in firm, parallel-shaped electrodes ideal for patch clamp experiments. HEATER 1 & 2:
These settings allow the user to display and adjust the specific heating values for each pulling stage on the digital LED readout. Operational Parameters
The final shape of the pipette is determined by three main variables:
Narishige PC-10 is a vertical micropipette puller that uses gravity and automated two-stage pulling to create glass needles for microinjection and patch clamping. Tritech Research Key Controls & Components Mode-Selector Knob : Switches between four modes: : Starts single-stage pulling. : Starts two-stage (double) pulling. NO. 1 HEATER / NO. 2 HEATER
: Displays and allows adjustment of heating values on the LED digital readout. Heater Level Adjusting Knobs : Sets digital values (0–100) for the heating intensity.
: Uses a combination of light (~25g) and heavy (~100g) weights to adjust pulling force. Adjustment Sliders
: Controls the "First Pull Terminating Position" and the total travel distance of the heater unit. ALA Scientific Operating Guide: Two-Stage Pulling
Two-stage pulling is typically used to create fine, sharp tips with reproducible tapers. BioMedical Instruments Preparation Turn on the Power Switch on the Mode-Selector Knob.
Install the desired number of weights. Using all four weights creates a sharper, finer tip; fewer weights create a longer, more gradual taper. Mounting the Glass
Raise the top electrode holder until it "clicks" into the first pull position. Narishige GD-1
(or equivalent 1.0mm O.D.) glass capillary through the heater coil.
Tighten the top and bottom holders, ensuring the capillary is perfectly centered in the heater coil to avoid off-center or distorted tips. The First Pull
Rotate the bottom switch 90 degrees counterclockwise to the "under weight" position (if applicable to your version). Press the red Start Switch
. The heater will activate, and the weight will drop a short distance (controlled by the adjustment slider) to thin the glass. The Second Pull
Once the first pull is done, center the filament holder on the now-thinned part of the tube.
Rotate the bottom switch 90 degrees clockwise to clear the weight path. Start Switch again. The unit will heat at the NO. 2 HEATER level and pull the glass until it separates. NARISHIGE WEB NEWS Reference Settings Examples Application First Pull Distance Thin/Long Pipette 3 (2L, 1H) Firm/Stubby Tip 4 (2L, 2H) Safety & Maintenance Tips No.051 Setting Values in the PC-10 Puller (Revised)
Summary of a successful pipette making◆ 1. Decide either One- or Two-stage pull, which is depended on what kind of pipette needed; NARISHIGE WEB NEWS Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS
Review: Narishige PC-10 Micropipette Puller (New Unit)
Rating: ★★★★½ (4.5/5)
The Bottom Line: The Narishige PC-10 is the "Toyota Hilux" of micropipette pullers—it isn't flashy, it lacks a digital touchscreen, and it looks like it was designed in 1995, but it is incredibly reliable, easy to fix, and produces consistent patches day in and day out. If you are tired of fighting with the programming menus of the Sutter P-97, this analog workhorse is a breath of fresh air.
Ease of Use & Setup: The biggest selling point for new users is the simplicity. Out of the box, setup took about 20 minutes. There is no complex software interface. You are greeted with two simple heater settings (Heater 1 and Heater 2) and a weight adjustment dial. For a lab transitioning from a broken older model, the learning curve is virtually non-existent. The "new" manual included is concise and actually helpful—unlike some translated technical manuals that are incomprehensible. The schematics for filament alignment were clear and accurate.
Performance: We use this primarily for standard patch-clamp electrodes (borosilicate glass, 1.5mm OD).
Build Quality: This is built like a tank. The mechanical solenoid is loud (a distinct CLACK sound), but it feels industrial and durable. The filament access is easy to open, making cleaning and filament replacement painless. The unit takes up a small footprint on the bench, which is a bonus for crowded electrophysiology rigs.
Pros:
Cons:
Final Verdict: If you need a "set it and forget it" puller for routine patch-clamping, the Narishige PC-10 is an excellent investment. It strips away the unnecessary digital complexity of modern pullers and focuses on the physics of melting glass. Highly recommended for labs that value reliability over bells and whistles. narishige pc10 manual new
Note: If by "PC10" you actually meant the PC-10 hydraulic micromanipulator or a specific accessory part, please clarify, but the above review applies to their most popular item bearing that model designation.
The Narishige PC-10 is a vertical dual-stage glass micropipette puller widely recognized for its versatility and straightforward gravity-based pulling method. Performance Review Summary Pros:
Versatility: Capable of producing both long, thin needles for injection and firm microelectrodes for patch clamping using single- or double-pull modes.
Ease of Use: Features a digital LED display for heater levels (0–100) and automated double-pull settings, which simplifies mass production compared to manual methods.
Stability: Uses a stabilized power supply to minimize output voltage variations, improving reproducibility.
Cost-Effective: Often noted as a more affordable and robust alternative to competing models like the Sutter P-30. Cons:
Variability: Users have reported variability in tip size and resistance, requiring frequent manual adjustments to maintain consistency.
Steep Learning Curve: Some experts suggest it is best suited for users who already understand pipette pulling mechanics due to the adjustment time required.
Tip Asymmetry: Because it is a vertical puller, the upper and lower pipettes often have slightly different shapes due to residual heat affecting the upper portion. Key Manual & Operational Highlights
Based on official PC-10 user documentation, here are the core settings you will manage: Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS
The story of the Narishige PC-10 is one of a legendary laboratory workhorse that served as the global standard for micropipette fabrication for decades before its retirement. Known for its "gravity-fall" vertical pulling method, it allowed researchers to create ultra-fine needles for microinjection and patch-clamp experiments with remarkable consistency. The Legacy of the PC-10
For years, the PC-10 was the go-to tool for electrophysiologists. It was designed to replace older models like the PP-830 and PB-7, offering a more automated, dual-stage system.
Precision Engineering: It used a stabilized power source to ensure the heating coil delivered the exact same amount of current every time, a critical feature for reproducibility.
Environmental Protection: A signature acrylic shroud shielded the glass capillaries from external air drafts, preventing even tiny temperature fluctuations from ruining a pull.
Customizable Pulls: Scientists would fine-tune their needles by swapping between two pairs of weights—light and heavy—to adjust the gravitational pulling force. The Transition to the "New" Manual (PC-100)
In 2017, Narishige officially discontinued the PC-10, replacing it with the PC-100. While the fundamental mechanics remain the same, the "new" manual for the PC-100 reflects several modern upgrades:
Improved Operability: The setup is faster, and the digital display now provides clearer status updates, including the elapsed time from start to finish.
Real-time Adjustments: Unlike the PC-10, the PC-100 allows you to adjust the heater level even after the needle has been placed in the coil.
Enhanced Safety: The acrylic cover on the newer model is designed to close more securely, and the internal slider unit is better protected from lab dust. Finding Manuals & Support
If you are looking for the original Narishige PC-10 Manual, it is still hosted by distributors like ALA Scientific Instruments for legacy support. For those upgrading, the PC-100 Product Page at Narishige Group provides documentation for the current standard.
Paper: Optimized Calibration and Operation of the Narishige PC-10 Vertical Puller 1. Introduction
The Narishige PC-10 is a high-precision vertical puller designed to produce long, thin microneedles or firm microelectrodes. It operates on a vertical axis, using the gravitational force of internal weights to pull heated glass. 2. Technical Specifications & Modes
Pulling Mechanism: Uses a combination of heater output and interchangeable weights (light and heavy). Operating Modes:
Single Pull: Pulls the capillary in one continuous stretch for specific needle shapes.
Double Pull: Changes heater or tension settings mid-process automatically, ideal for patch pipettes.
Materials: Compatible with borosilicate glass capillaries, such as the Narishige GD-1 with internal filaments. 3. Calibration & Setup (Manual Guidelines)
Proper setup is critical for reproducibility. Users often reference technical guides from NARISHIGE WEB NEWS to determine starting values.
Heater Values: The display shows digital values (0–100) representing output power.
Weight Adjustment: Changing the number of weights (e.g., using 2 vs. 4 weights) drastically alters the pipette’s angle and parallelism.
Environmental Compensation: Pulling temperatures must be adjusted based on ambient lab temperature and the "age" of the heating filament. 4. Operational Best Practices
Expert users on platforms like ResearchGate suggest specific workflows:
Filament Maintenance: Replace filaments every 1–2 years or when heating becomes inconsistent.
Contact Cleaning: If heater function is irregular, polish the contact surfaces between the heater block and mounting plates with sandpaper. Overall: 7
Two-Step Refinement: Use a high first-stage temperature to initiate melting and a lower second-stage temperature to refine the tip resistance. 5. Maintenance and Troubleshooting
Error Handling: If the slider falls prematurely in two-stage mode, check the First Pull Terminating Position Adjusting Slider and ensure the solenoid coil is functioning.
Filament Care: Ensure heater coils do not touch; use a wrench to separate them or replace the unit if the heater is deteriorated. 6. Conclusion
The PC-10 remains a reliable standard for microelectrode fabrication. Its versatility is maximized through precise weight adjustment and the automated double-pull mode, provided the user performs regular Heater Block maintenance.
Report: Narishige PC-10 Manual - New
Introduction
The Narishige PC-10 is a precision microinjector designed for use in various scientific research applications, particularly in the fields of biology, medicine, and biotechnology. This report provides an overview of the Narishige PC-10 manual, focusing on the new features, operating instructions, and technical specifications.
Overview of Narishige PC-10
The Narishige PC-10 is a computer-controlled microinjector that allows for precise and controlled injection of liquids into cells or tissues. It is widely used in research applications such as intracellular recording, drug delivery, and gene injection.
New Features in the Updated Manual
The updated Narishige PC-10 manual includes the following new features:
Operating Instructions
The Narishige PC-10 manual provides step-by-step instructions on how to operate the device:
Technical Specifications
The Narishige PC-10 manual provides detailed technical specifications, including:
Safety Precautions
The Narishige PC-10 manual highlights the importance of safety precautions when operating the device:
Conclusion
The updated Narishige PC-10 manual provides a comprehensive guide to the operation, technical specifications, and safety precautions of the device. The new features and instructions outlined in the manual will help researchers to optimize their use of the device and achieve accurate and reliable results.
Recommendations
Based on the information provided in the Narishige PC-10 manual, we recommend that users:
A standout feature for the Narishige PC-10 Go to product viewer dialog for this item.
(often referred to as a manual or vertical puller) is its automated dual-stage pulling mode, which significantly improves the reproducibility of specialized pipette tips.
While simple in design, this system allows you to preset two different heating levels—one for the initial stretch and another for the final pull—enabling the production of everything from long, thin microinjection needles to firm, blunt microelectrodes for patch-clamping. Key Specifications & Features
Gravity-Fed Tensioning: It uses a classic vertical "free fall" method with two pairs of interchangeable weights (two light and two heavy) to provide consistent pulling force without the complexity of electromagnetic coils.
Stabilized Power Supply: The unit includes a built-in power source designed to minimize voltage variations, ensuring the heater element delivers uniform current for every pull.
Digital LED Display: A clear readout shows the exact heater level status, making it easy to mass-produce pipettes with identical tip geometries once you've found your ideal settings.
Protective Acrylic Shroud: An integrated cover shields the glass capillary from air currents and drafts during the pulling process, preventing external environmental factors from affecting the final tip shape.
Fine Control: The heater control uses a ten-turn adjusting knob for ultra-precise settings, allowing for tip sizes ranging from several tens of micrometers down to sub-micron levels.
Detailed operational settings and maintenance tips can be found in the Narishige PC-10 User Manual.
The Narishige PC-10 is a versatile, vertical-pull micropipette puller designed for the high-precision fabrication of glass microelectrodes and injection needles. This model replaces older semi-automatic units like the PP-830, offering a more automated and robust experience for researchers in biotechnology and electrophysiology. Core Operation Modes
The PC-10 features two primary pulling modes that determine the final shape and utility of the pipette:
Single-Stage (Step 1): Pulls the glass capillary in one continuous motion. This mode typically produces larger tip diameters or more "supple" pipettes. used for sharp intracellular electrodes.
Two-Stage (Step 2): Pulls the capillary in two separate heat-and-pull cycles. This is ideal for creating firm, parallel microelectrodes for patch-clamping or long, thin needles for microinjection. Precision Controls and Settings
The device uses gravitational force as its pulling mechanism, which can be adjusted through several manual and digital controls:
Weight Blocks: Tension is adjusted by adding or removing weights. Two types (light ~25g and heavy ~100g) are provided to allow for four distinct levels of pulling force.
Digital Heater Levels: Values from 0 to 100 represent the proportion of maximum power output (approx. 2.5V) delivered to the Kanthal wire heating element.
Adjustment Plates: In two-stage mode, researchers can set the "drop length" for the first pull to determine where the second heating phase begins, which directly influences the tip's final taper and sharpness. Critical Maintenance and Calibration
According to the Narishige PC-10 User Manual, achieving reproducibility requires careful alignment and environmental awareness:
Centering: For consistent results, the glass capillary must be perfectly centered within the heating coil. Users should loosen the heating element positioning screw to align the heater block before tightening.
Environmental Factors: Because the unit relies on radiant heat, external factors like air currents and humidity can affect performance. The PC-10 includes an acrylic shroud to shield the pipette from drafts.
Heater Integrity: If a pipette fails to separate or looks distorted, the heater coil may be bent or deteriorated. Replacing a heater involves loosening copper clamps and using a piece of glass as a guide to ensure the new coil is correctly centered. Troubleshooting Tips No.051 Setting Values in the PC-10 Puller (Revised)
Narishige PC-10 is a vertical micropipette puller that uses gravity and controlled heating to create glass needles for microinjection or electrophysiology. While the newer
has improved digital displays, the core mechanics of the PC-10 remain the standard for reliable, manual pipette production. NARISHIGE GROUP Quick Setup Guide 1. Control Panel Overview Power Switch: Located on the front; the Heater Level display illuminates when on. Mode-Selector Knob: Single-stage pulling (one continuous pull).
Two-stage pulling (automated change in heating/tension mid-process). No. 1 Heater: Used to manually test and set the heating value. Start Switch: Initiates the pulling sequence. ALA Scientific 2. Adjusting the Pull Characteristics
Pipette shape is determined by the balance of heat and weight: NARISHIGE WEB NEWS Heater Level:
Higher values (max 100) melt more glass, leading to longer, thinner tips.
The unit includes two light (approx. 25g) and two heavy (approx. 100g) weights. More Weight: Results in a steeper taper and sharper edge. Less Weight: Results in a thinner, more parallel tip. NARISHIGE WEB NEWS 3. Step-by-Step Operation Mount the Capillary: Place a glass capillary (e.g., ) into the heater coil and secure it with the clamps. Select Mode: for simple needles or for firm microelectrodes used in patch clamping. Adjust Heating: No. 1 Heater knob to your desired value (start around 60 for reference). Set Tension:
Add or remove weights from the slider based on the required tip sharpness.
switch. The unit will automatically release the weights once the glass reaches the melting point. NARISHIGE WEB NEWS Maintenance & Safety Tips Heater Safety:
Never touch the heater unit while the power is on; it retains residual heat for 5–10 minutes after being switched off. Filament Care:
If the heater coil becomes brittle or fails to heat (Error E_99), it must be replaced. Environment:
Consistency can vary with lab humidity; keep a log of settings that work for your specific environment. ResearchGate
For detailed technical troubleshooting, you can refer to the Narishige FAQ page or download the full PC-10 PDF manual weight and heat settings
for specific pipette types, like patch-clamp vs. injection needles? AI responses may include mistakes. Learn more No.051 Setting Values in the PC-10 Puller (Revised)
Subject: Narishige PC-10 Manual: A Comprehensive User Guide and Technical Overview
The PC-10 has several mechanical components that wear out: the heater base, the solenoid plunger, the micro-switch, and the braking mechanism. A new manual should provide an exploded view with Narishige part numbers (e.g., for the heating filament or O-rings).
Yes, buy if:
No, avoid if:
The Narishige PC-10 isn’t just a puller; it’s a time capsule of mechanical genius. Before digital touchscreens and software updates, the PC-10 used gravity, a magnetic solenoid, and a carefully calibrated heater to pull glass capillaries into fine-tipped micropipettes. No firmware crashes. No DRM. Just physics and skill.
Yet, when a lab inherits a PC-10 today—often bought second-hand from an online auction or passed down by a retiring professor—the manual is almost always missing. And without it, the machine is a silent metal box with cryptic knobs labeled HEATER, MAGNET, and TIMER.
Technically, yes. Practically, no. The PC-10 is deceptively simple. Without the manual, here is what typically goes wrong:
A new manual is not just paperwork—it is a safety device. If your lab still uses a PC-10, print a fresh copy and laminate the calibration page next to the machine.
The PC-10 allows for single-stage or two-stage pulling. The standard method for general-purpose intracellular electrodes is usually a single stage, while patch-clamp electrodes often benefit from a two-stage approach.
A. Setting the Heating Power
B. The Single-Stage Pull This is the simplest method, used for sharp intracellular electrodes.
C. The Two-Stage Pull (for Patch Clamp Electrodes) This method allows for control over the "shank" length and tip size.