MIDV‑075 possesses a single‑stranded, positive‑sense RNA genome of 10,972 nucleotides. It encodes a polyprotein that is proteolytically cleaved into five mature proteins:
| Gene | Approx. Length (aa) | Putative Function | |------|----------------------|-------------------| | C (Capsid) | 272 | Nucleocapsid formation, RNA packaging | | prM/M | 426 | Membrane protein, virion assembly | | E (Envelope) | 516 | Host‑cell receptor binding, membrane fusion | | NS1 | 352 | Immune evasion, complement antagonism | | NS2‑NS5 (non‑structural cluster) | 1,940 | Replication complex (RNA‑dependent RNA polymerase, helicase, methyltransferase) | MIDV-075
A notable feature is the presence of a conserved “KDEL” motif at the C‑terminus of NS1, a hallmark of flaviviral secreted proteins, yet the upstream protease cleavage sites resemble those of orthobunyaviruses. This mosaic pattern underscores the virus’s evolutionary chimerism, likely a product of ancient recombination events between divergent arboviral lineages. Rectification:
Beyond Vero and DF‑1 cells, MIDV‑075 replicates—albeit at lower efficiency—in: Beyond Vero and DF‑1 cells
These results indicate a broad cellular tropism that includes both vertebrate and invertebrate hosts. The virus exploits the DC‑SIGN receptor on mammalian cells—a mechanism shared with several flaviviruses—while entry into avian cells appears to rely on a yet‑unidentified sialic‑acid–linked receptor.