For patients:
For institutions building a MET CN:
Carbon and nitrogen are core macronutrients. Cells coordinate assimilation, storage, and utilization of carbon (C) and nitrogen (N) through multilayered regulation—transcriptional, translational, post-translational, and allosteric. Imbalances affect growth, stress tolerance, and productivity. This paper synthesizes current knowledge and proposes experimental approaches to probe MET-CN dynamics. For patients:
The golden age of METCN coincided with the explosion of broadband internet in China. During this period, several distinct "brands" or "series" under the METCN umbrella gained cult status: For institutions building a MET CN:
MET-CN is orchestrated by sensing mechanisms that monitor metabolite levels (e.g., 2-oxoglutarate as a C/N signal), energy status (AMP/ATP), and amino acid pools. Signaling hubs (TOR, AMPK, GCN2) integrate inputs to modulate anabolic/catabolic balance. Engineering strategies: modify 2-oxoglutarate sensors, optimize glutamine synthetase regulation, or rewire transporters to improve nitrogen use efficiency in crops. Challenges include compartmentalization and temporal dynamics. Carbon and nitrogen are core macronutrients